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Catalog Number | orb2292476 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a full length recombinant ORM1. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 2F9-1F10 |
Tested applications | ELISA, IHC-P, IP, WB |
Reactivity | Human |
Isotype | IgG1 kappa |
Immunogen | ORM1 (AAH26238, 18 a.a. ~ 201 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugation | Unconjugated |
Protein Sequence | AQIPLCANLVPVPITNATLDRITGKWFYIASAFRNEEYNKSVQEIQATFFYFTPNKTEDTIFLREYQTRQDQCIYNTTYLNVQRENGTISRYVGGQEHFAHLLILRDTKTYMLAFDVNDEKNWGLSVYADKPETTKEQLGEFYEALDCLRIPKSDVVYTDWKKDKCEPLEKQHEKERKQEEGES |
NCBI | AAH26238 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Detection limit for recombinant GST tagged ORM1 is approximately 0.1 ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to ORM1 on formalin-fixed paraffin-embedded human stomach carcinoma tissue. [antibody concentration 1 ug/ml]
Immunoprecipitation of ORM1 transfected lysate using anti-ORM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ORM1 MaxPab rabbit polyclonal antibody.
ORM1 monoclonal antibody (M01), clone 2F9-1F10 Western Blot analysis of ORM1 expression in MCF-7.
Western Blot analysis of ORM1 expression in transfected 293T cell line by ORM1 monoclonal antibody (M01), clone 2F9-1F10. Lane 1: ORM1 transfected lysate (23.5 KDa). Lane 2: Non-transfected lysate.
Western blot analysis of ORM1 over-expressed 293 cell line, cotransfected with ORM1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ORM1 monoclonal antibody (M01), clone 2F9-1F10 (Cat # orb2292476). GAPDH (36.1 kDa) used as specificity and loading control.
Western Blot detection against Immunogen (45.98 KDa).