ORF9c Antibody

• Catalog Number: orb1239056
• Category: Antibodies
• Description: ORF9c Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Virus
• Isotype: IgG
• Immunogen: Anti-SARS-CoV-2 (COVID-19) ORF9c antibody (orb1239056) was raised against a peptide corresponding to 16 amino acids near the amino-terminus of SARS-CoV-2 (COVID-19) ORF9c protein. The immunogen is located within the first 50 amino acids of the SARS-CoV-2 (COVID-19) ORF9c protein.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.25-1 μg/mLAntibody validated: SARS-CoV-2 (COVID-19) ORF9c antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. It can detect SARS-CoV-2 ORF9c in transfected human cells by WB. All other applications and species not yet tested
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 8kD
• Target: ORF9c
• UniProt ID: P0DTD3
• NCBI: P0DTD3
• Storage: SARS-CoV-2°COVID-19) ORF9c antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: SARS-CoV-2 (COVID-19) ORF9c antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: ORF9c protein, Uncharacterized protein 14, ORF14,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation with SARS-CoV-2 (COVID-19) ORF9c Overexpressed 293 Cells. Loading: 10 µg per lane of 293 cell lysate from WT and SARS-CoV-2 (COVID-19) ORF9c transfected cells. Antibodies: SARS-CoV-2 (COVID-19) ORF9c orb1239056, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation with SARS-CoV-2 (COVID-19) ORF9c Overexpressed 293 Cells. Loading: 10 µg per lane of 293 cell lysate from WT and SARS-CoV-2 (COVID-19) ORF9c transfected cells. Antibodies: SARS-CoV-2 (COVID-19) ORF9c, orb1239056, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 µg/mL and. Lane 2: 0.5 µg/mL.

ELISA Validation. Antibodies: SARS-CoV-2 (COVID-19) ORF9c Antibody, orb1239056. A direct ELISA was performed using SARS-CoV-2 ORF9c immunogen peptide (orb1231666) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF9c antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL.