ORF3b Antibody

• Catalog Number: orb1239062
• Category: Antibodies
• Description: ORF3b Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Virus
• Isotype: IgG
• Immunogen: Anti-SARS-CoV-2 (COVID-19) ORF3b antibody (orb1239062) was raised against a peptide corresponding to 16 amino acids near the central domain of SARS-CoV-2 (COVID-19) ORF3b protein. The immunogen is located within 30-80 amino acids of the SARS-CoV-2 (COVID-19) ORF3b protein.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.25 μg/mL; IHC: 0.5 μg/mL Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) ORF3b antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. It can detect SARS-CoV-2 ORF3b recombinant protein by ELISA and WB. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 17kD
• Target: ORF3b
• UniProt ID: P0DTC5
• NCBI: P0DTC3
• Storage: SARS-CoV-2°COVID-19) ORF3b antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: SARS-CoV-2 (COVID-19) ORF3b antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: ORF3b protein, Acessary protein 3b, ns3b, Non-stru
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) ORF3b in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) ORF3b antibody (orb1239062, 0.5 µg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 ORF3b protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.

ELISA Validation with SARS-CoV-2 (COVID-19) ORF3b Protein. Antibodies: SARS-CoV-2 (COVID-19) ORF3b Antibody, orb1239062. A direct ELISA was performed using SARS-CoV-2 ORF3b recombinant protein (orb1231625) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF3b antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL.

Western Blot Validation with SARS-CoV-2 (COVID-19) ORF3b Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) ORF3b recombinant protein (orb1231625). Antibodies: SARS-CoV-2 (COVID-19) ORF3b, orb1239062, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 µg/mL and. Lane 2: 0.5 µg/mL.