ORAI1 Antibody

• Catalog Number: orb1239704
• Category: Antibodies
• Description: ORAI1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3F6H5
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: Mouse monoclonal ORAI1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ORAI1.
• Concentration: 1 mg/mL
• Dilution range: ORAI1 antibody can be used for detection of ORAI1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 33 kDa Observed: 56 kDa
• Target: ORAI1
• UniProt ID: Q96D31
• NCBI: Q96D31
• Storage: ORAI1 monoclonal antibody can be stored at -20°C, stable for one year.
• Buffer/Preservatives: ORAI1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: ORAI1 Antibody [3F6H5] : IMD9, ORAT1, CRACM1, TMEM
• Note: For research use only
• Application notes: ORAI1 antibody can be used for detection of ORAI1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at (A) 1 and (B) 2 μg/mL.

Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 5 μg/mL.

Immunofluorescence of ORAI1 in human spleen tissue with ORAI1 antibody at 20 μg/ml.

Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 2.5 μg/mL.

Immunofluorescence of ORAI1 in human spleen tissue with ORAI1 antibody at 20 μg/ml. Red: ORAI1 Antibody [3F6H5] (orb1239704) Blue: DAPI staining