OPAL1 antibody

• Catalog Number: orb222816
• Category: Antibodies
• Description: Mouse monoclonal antibody to OPAL1
• Clonality: Monoclonal
• Clone Number: OPAL1-01
• Tested applications: FC, WB
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: Recombinant fragment of human OPAL1 (amino acids 152-342)
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-4 µg/ml, intracellular staining.Western blotting: Recommended dilution: 1-2 μg/ml, non-reducing conditions.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: OPAL1
• Entrez: 54838
• UniProt ID: Q9NX94
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Alternative names: Anti-WBP1L antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis of OPAL-transfected HEK293 cells using mouse monoclonal antibody OPAL1-01.

Western blotting analysis of human OPAL1 using mouse monoclonal antibody OPAL1-01 on lysates of HEK-293T/OPAL1 transfectants, and of murine splenocytes and HEK-293T cells (negative controls) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-OPAL1 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. OPAL1 was detected at around 45-60 kDa.