ONPG

SKU: orb1307005

Description

ONPG is a chromogenic substrate for detecting β-galactosidase activity, widely used in molecular biology and biochemistry research. It enables the study of gene expression (e.g., lacZ reporter assays) in both in vitro enzymatic studies and in vivo cellular models.

Key Properties

• CAS Number: 369-07-3
• MW: 301.25
• Purity: 99.91%
• Formula: C₁₂H₁₅NO₈
• SMILES: O([C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O)C2=C(N(=O)=O)C=CC=C2
• Target: Others
• Solubility: H2O:7.4 mg/mL (24.56 mM)

Bioactivity

• Target IC50:
  β-gal:2.7±0.3 mM(Km)|β-gal:464.7±7.8/s(kcat)
• In Vitro:
  The enzyme displays high hydrolysis ability for ONPG (100%) and moderate activity for its natural substrate lactose (25.7%). However, the hydrolysis ability of the enzyme towards all other chromogenic nitrophenyl analogues is very weak, indicating that Gal308 is a β-galactosidase with narrow substrate specificity. To investigate the kinetic parameters of recombinant enzyme, the Michaelis-Menten constants (Km), turnover numbers (kcat), and catalytic efficiencies (kcat/Km) of Gal308 for ONPG and lactose are determined. The kcat and Km values are 464.7±7.8 s-1 and 2.7±0.3 mM for ONPG, and 264.2±2.1 s-1 and 7.1±0.8 mM for lactose, respectively. The kcat/Km value of the enzyme for ONPG (172.1 s-1mM-1) is 4.6-fold higher than that for lactose (37.2 s-1mM-1), which clearly demonstrated that the catalytic efficiency of Gal308 for ONPG is much higher than that for lactose.

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

2-Nitrophenyl β-D-galactopyranoside, 2-Nitrophenyl b-D-galactopyranoside, 2-Nitrophenyl beta-D-galactopyranoside, inhibit, Inhibitor, ONPG, β-gal

Compound Identifiers

PubChem CID

96647