ONECUT1 Antibody

• Catalog Number: orb1676272
• Category: Antibodies
• Description: ONECUT1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, WB
• Reactivity: Human
• Immunogen: E.coli-derived human ONECUT1 recombinant protein (Position: M1-E270).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.25-0.5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human Direct ELISA, 0.1-0.5 μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 50 kDa
• UniProt ID: Q9UBC0
• Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.

IF analysis of ONECUT1 and Tubulin beta using anti-ONECUT1 antibody;and anti-Tubulin beta antibody.ONECUT1 and Tubulin beta was detected in an immunocytochemical section of U2OS cells.

Western blot analysis of ONECUT1 using anti-ONECUT1 antibody.

Flow Cytometry analysis of Daudi cells using anti-ONECUT1 antibody(Blue line).Isotype control antibody(Green line) was rabbit IgG.Unlabelled sample(Red line) was also used as a control.