You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb570346 |
---|---|
Category | Antibodies |
Description | Oct4/POU5F1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human Oct4/POU5F1 recombinant protein (Position: E26-A300). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Flow Cytometry, 1-3μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 39 kDa |
UniProt ID | Q01860 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | POU domain, class 5, transcription factor 1; Octam Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of 293T cells using anti-POU5F1 antibody (orb570346). Overlay histogram showing 293T cells stained with orb570346 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POU5F1 Antibody (orb570346, 1μg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of HepG2 cells using anti-POU5F1 antibody (orb570346). Overlay histogram showing HepG2 cells stained with orb570346 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POU5F1 Antibody (orb570346, 1μg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
ICC, IF, IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Monoclonal | |
Unconjugated |
Filter by Rating