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Oct4 Antibody / POU5F1
Catalog Number: orb1151467
| Catalog Number | orb1151467 |
|---|---|
| Category | Antibodies |
| Description | Rabbit monoclonal antibody to OCT4 |
| Clonality | Recombinant |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG, kappa |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
| Purity | Protein A/G affinity |
| Immunogen | A portion of amino acids1-134 was used as the immunogen for the recombinant Oct4 antibody. |
| UniProt ID | Q01860 |
| Tested applications | IHC-P |
| Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml |
| Application notes | Optimal dilution of the recombinant antibody should be determined by the researcher. |
| Antibody Type | Primary Antibody |
| Clone Number | OCT4/6875R |
| Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Storage | Aliquot the recombinant antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Hazard Information | This recombinant Oct4 antibody is available for research use only. |
| Note | For research use only |
IHC staining of FFPE human testis with recombinant Oct4 antibody (clone OCT4/6875R). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human seminoma tissue with recombinant Oct4 antibody (clone OCT4/6875R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human seminoma tissue with recombinant Oct4 antibody (clone OCT4/6875R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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