OCT-1 Mouse Monoclonal Antibody

SKU: orb500699

Description

OCT-1 Mouse Monoclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 9A3
• Immunogen: KLH conjugated synthetic peptide derived from human Oct1
• Target: POU2F1
• Molecular Weight: 76 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

class 2; transcription factor 1; FLJ42836; NF A1; NF-A1; Oct1; Oct-1; OCT1; Octamer binding protein 1; Octamer binding transcription factor 1; Octamer-binding protein 1; Octamer-binding transcription factor 1; OTF 1; OTF-1; OTF1; OTTHUMP00000032348; OTTHUMP00000032350; OTTHUMP00000032351; PO21; PO2F1; PO2F1_HUMAN; POU class 2 homeobox 1; POU domain; POU domain class 2 transcription factor 1; POU2F1.

Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (OCT-1) Polyclonal Antibody, Unconjugated (orb500699) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-OCT-1 (orb500699) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 76 kD, Observed band size: 80 kD.

Sample: Raji (Human) Cell Lysate at 30 ug, Primary: Anti-OCT-1 (orb500699) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 76 kD, Observed band size: 80 kD.

Quick Database Links

Gene Symbol

POU2F1

UniProt

P14859