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Catalog Number | orb2291304 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a partial recombinant NUP62. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 2D3 |
Tested applications | ELISA, IF, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG2b Kappa |
Immunogen | NUP62 (NP_714941, 423 a.a. ~ 522 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugation | Unconjugated |
Protein Sequence | LQHADEEREKTYKLAENIDAQLKRMAQDLKDIIEHLNTSGAPADTSDPLQQICKILNAHMDSLQWIDQNSALLQRKVEEVTKVCEGRRKEQERSFRITFD |
NCBI | NP_714941 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Detection limit for recombinant GST tagged NUP62 is approximately 1 ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to NUP62 on HeLa cell. [antibody concentration 10 ug/ml]
NUP62 monoclonal antibody (M02), clone 2D3 Western Blot analysis of NUP62 expression in Hela S3 NE.
NUP62 monoclonal antibody (M02), clone 2D3. Western Blot analysis of NUP62 expression in NIH/3T3.
NUP62 monoclonal antibody (M02), clone 2D3. Western Blot analysis of NUP62 expression in PC-12.
Western Blot analysis of NUP62 expression in transfected 293T cell line by NUP62 monoclonal antibody (M02), clone 2D3. Lane 1: NUP62 transfected lysate (Predicted MW: 53.3 KDa). Lane 2: Non-transfected lysate.
Western Blot detection against Immunogen (36.74 KDa).