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Catalog Number | orb390196 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to NUP35. |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human NUP35 (NP_612142.2). |
Dilution range | WB: 1:500-2000 |
Conjugation | Unconjugated |
MW | 37kDa |
UniProt ID | Q8NFH5 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
Alternative names | NUP35, MP-44, MP44, NP44, NUP53 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of various lysates using NUP35 Rabbit pAb (orb390196) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
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