Nucleoside phosphorylase/PNP Antibody

• Catalog Number: orb389429
• Category: Antibodies
• Description: Nucleoside phosphorylase/PNP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PNP (161-189aa AMSDAYDRTMRQRALSTWKQMGEQRELQE), different from the related mouse sequence by six amino acids, and from the related rat sequence by five amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 32118 MW
• UniProt ID: P00491
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Purine nucleoside phosphorylase;PNP;2.4.2.1;Inosin
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U251 cells using anti-PNP antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of U937 cells using anti-PNP antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of PNP using anti-PNP antibody.Lane 1:human 293T cell;2:human PC-3 cell; 3:rat liver tissue.

IHC analysis of PNP using anti-PNP antibody.PNP was detected in paraffin-embedded section of mouse kidney tissues.

IHC analysis of PNP using anti-PNP antibody.PNP was detected in paraffin-embedded section of rat kidney tissues.

IHC analysis of PNP using anti-PNP antibody.PNP was detected in paraffin-embedded section of human renal cancer tissues.

IHC analysis of PNP using anti-PNP antibody.PNP was detected in paraffin-embedded section of human liver cancer tissues.