Nucleolin/NCL Antibody

• Catalog Number: orb1529757
• Category: Antibodies
• Description: Nucleolin/NCL Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: between 525 and 575
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Nucleolin/NCL
• UniProt ID: P19338
• NCBI: NP_005372.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: C23; Protein C23; Nsr1; nucleolin
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Nucleolin by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-Nucleolin used at a dilution of 1:200 (1µg/ml).

Detection of human Nucleolin by WB and IP.Samples: HeLa, HEK293T and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-Nucleolin antibody used for WB at 0.02 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B).