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Catalog Number | orb257222 |
---|---|
Category | Proteins |
Description | Nuclease DMF Filed Protein |
Reactivity | Bacteria |
Tag | Native |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 26.8 kDa |
Target | Nuclease |
Source | Nuclease (orb257222) is expressed from E. coli cells. |
Expression System | E. coli |
Endotoxins | 1.0 EU per μg |
Storage | -20°C |
Buffer/Preservatives | Tris HCl, pH 8.0, MgCl2, and NaCl |
Alternative names | Nuclease,BenzNuclease,BenzoNuclease,benzonase,Nucl Read more... |
Note | For research use only |
Application notes | BenzNuclease is a recombinant form of Serratia macescens extracellular endonuclease produced in Escherichia coli cells using a proprietary process at ACRObiosystems. BenzNuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form ( single stranded, double stranded, linear, circular and supercoiled ). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5'-phosphorylated oligonucleotides of 3-8 bases in length. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of BenzNuclease protein
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