Nuclear respiratory factor 1 NRF1 Antibody

• Catalog Number: orb97074
• Category: Antibodies
• Description: Nuclear respiratory factor 1 NRF1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human NRF1 (272-288aa QHGREDLLYAFEDQQTQ), identical to the related rat and mouse sequences.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, RatImmunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By HeatWestern blot, 0.1-0.5μg/ml, Human, Rat, Mouse
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 53541 MW
• UniProt ID: Q16656
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Nuclear respiratory factor 1;NRF-1;Alpha palindrom
• Note: For research use only
• Application notes: WB: The detection limit for NRF1 is approximately 2.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Confocal immunofluoroscence analysis of A549 cells using NRF1 antibody

Western blot analysis of Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Kidney Tissue Lysate, Lane 3: MCF-7 Cell Lysate, Lane 4: A549 Cell Lysate using NRF1 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Human Mammary Cancer Tissue using NRF1 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Intestine Tissue using NRF1 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Intestinal Cancer Tissue using NRF1 antibody