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Catalog Number | orb1528272 |
---|---|
Category | Antibodies |
Description | NUCKS Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP |
Reactivity | Human |
Immunogen | between 147 and 197 |
Concentration | 1000 µg/ml |
Dilution range | WB - Not recommended. A301-508A has not performed satisfactorily when used for WB of NUCKS in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of NUCKS.; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | NUCKS |
UniProt ID | Q9H1E3 |
NCBI | NP_073568.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | nuclear ubiquitous casein kinase and cyclin-depend Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human NUCKS by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-NUCKS used at a dilution of 1:5, 000 (0.2µg/ml).
Detection of human NUCKS by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-NUCKS antibody.
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