NTF3 Antibody

• Catalog Number: orb1272350
• Category: Antibodies
• Description: NTF3 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, NeA, WB
• Reactivity: Human
• Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hNT-3 (human Neurotrophin-3).
• Concentration: batch dependent
• Dilution range: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hNT-3 (50.00 ng/mL), a concentration of 2.0 - 4.0 μg/mL of this antibody is required. ELISA:To detect hNT-3 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hNT-3. Sandwich:To detect hNT-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human NT-3as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNT-3. Western Blot:To detect hNT-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNT-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: NTF3
• UniProt ID: P20783
• NCBI: P20783
• Storage: NT-3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: NT3, HDNF, NGF2, NT-3, NGF-2Neurotrophin-3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.