NTAL antibody

• Catalog Number: orb44460
• Category: Antibodies
• Description: Mouse Monoclonal to NTAL.
• Clonality: Monoclonal
• Clone Number: NAP-07
• Tested applications: FC, ICC, IP, WB
• Reactivity: Human, Mouse
• Isotype: Mouse IgG1
• Immunogen: Recombinant cytoplasmic domain (aa 91-243) of human NTAL.
• Concentration: 1 mg/ml
• Dilution range: Western blotting: Recommended dilution: 1-2 μg/ml.Flow cytometry: Intracellular staining; recommended dilution: 1-4 µg/ml; positive tissue: RAJI human lymphoma cell line, RAMOS human lymphoma cell line.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: NTAL
• Entrez: 56743
• UniProt ID: Q9JHL0
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: NTAL antibody, anti-LAB
• Note: For research use only
• Application notes: Western blotting: Recommended dilution: 2 μg/ml, incubation 60 min; positive control: RAMOS human lymphoma cell line, negative control: HeLa human cervix carcinoma cell line; non-reducing conditions, 12% separating SDS-PAGE gel. Flow cytometry: Intracellular staining; recommended dilution: 1-4 µg/ml; positive tissue: RAJI human lymphoma cell line, RAMOS human lymphoma cell line.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-NTAL (NAP-07) purified antibody (concentration in sample 9 µg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (20 µl reagent / 100 µl of peripheral whole blood).

Separation of human CD3 negative NTAL positive lymphocytes (red-filled) from CD3 positive NTAL negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-NTAL (NAP-07) purified antibody (concentration in sample 9 µg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-NTAL (NAP-07) purified antibody (concentration in sample 9 µg/ml, GAM APC).

Immunocytochemistry analysis of NTAL by confocal microscopy in THP-1 human acute monocytic leukemia cell line. The cells were permeabilized and immunostained using anti-NTAL (NAP-07; green). Nuclei are vizualized by propidium iodide (red).

Western blotting analysis of human NTAL using mouse monoclonal antibody NAP-07 on lysates of Raji and Ramos cells and on Jurkat cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. NTAL was detected around 27 kDa.