NT5DC2 antibody

• Catalog Number: orb312336
• Category: Antibodies
• Description: NT5DC2 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Canine, Equine, Mouse, Porcine, Rabbit, Sheep
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human NT5DC2 (251-350/520 aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 61 kDa
• Target: NT5DC2
• UniProt ID: Q9H857
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: 5' nucleotidase domain containing 2; 5'-nucleotida
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Cui, Yuying et al. Decreased RNA?binding protein IGF2BP2 downregulates NT5DC2, which suppresses cell proliferation, and induces cell cycle arrest and apoptosis in diffuse large B?cell lymphoma cells by regulating the p53 signaling pathway Mol Med Rep, 26, 286 (2022)

PubMed: 35894142

Applications

WB

Reactivity

Human

Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30

Sample: HT1080(Human) Cell Lysate at 30 ug Primary: Anti-NT5DC2 (orb312336) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kD Observed band size: 61 kD