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Catalog Number | orb1239075 |
---|---|
Category | Antibodies |
Description | NSP16 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Virus |
Isotype | IgG |
Immunogen | Anti-SARS-CoV-2 (COVID-19) NSP16 antibody (orb1239075) was raised against a peptide corresponding to 16 amino acids near the central domain of SARS-CoV-2 (COVID-19) NSP16 protein. The immunogen is located within 120-170 amino acids of the SARS-CoV-2 (COVID-19) NSP16 protein. |
Concentration | 1 mg/mL |
Dilution range | IHC: 0.5 μg/mL; WB: 0.5 μg/mLAntibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) NSP16 antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. It can detect SARS-CoV-2 NSP16 recombinant protein by ELISA and WB. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | NSP16 |
UniProt ID | P0DTC1 |
NCBI | YP_009725311 |
Storage | SARS-CoV-2°COVID-19) NSP16 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | SARS-CoV-2 (COVID-19) NSP16 antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | 2'-O-methyltransferase, nsp16, Non-structure prote Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) NSP16 in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) NSP16 antibody (orb1239075, 0.5 µg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP16 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
ELISA Validation with SARS-CoV-2 (COVID-19) NSP16 Protein. Antibodies: SARS-CoV-2 (COVID-19) NSP16 Antibody, orb1239075. A direct ELISA was performed using SARS-CoV-2 NSP16 recombinant protein (orb1231572) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP16 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL.
Western Blot Validation with SARS-CoV-2 (COVID-19) NSP16 Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) NSP16 recombinant protein (orb1231572). Antibodies: SARS-CoV-2 (COVID-19) NSP16, orb1239075, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 µg/mL and. Lane 2: 2 µg/mL.
ELISA | |
Virus | |
Rabbit | |
Polyclonal | |
Unconjugated |
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