NSE Recombinant Rabbit Monoclonal Antibody

SKU: orb1816767

Description

NSE Recombinant Rabbit Monoclonal Antibody

Research Area

Cancer Research, Cell Biology, Energy Metabolism, Metabolism, Tumor Biology

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IF=1:50-200, IHC-P=1:50-200, IHC-F=1:50-200
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: H12A5
• Immunogen: A synthesized peptide derived from human gamma Enolase (1-60/434aa)
• Target: ENO2
• Molecular Weight: 48 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS, Glycerol, rAlbumin.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

gamma-enolase; gamma enolase; Neural enolase; Neuron specific enolase; neurone-specific enolase; 2 phospho D glycerate hydrolyase; Eno 2; Eno2; ENO2; ENOG; Enolase 2; Enolase 2 gamma neuronal; Enolase2; Neuron specific enolase; Neuron specific gamma enolase; NSE; ENOG_HUMAN; gamma-enolase; 2-phospho-D-glycerate hydro-lyase; enolase 2, gamma, neuronal.

25 ug total protein per lane of various lysates probed with NSE monoclonal antibody, unconjugated (orb1816767) at 1:3000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with NSE Monoclonal Antibody, Unconjugated (orb1816767) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Cerebellum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with NSE Monoclonal Antibody, Unconjugated (orb1816767) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with NSE Monoclonal Antibody, Unconjugated (orb1816767) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with NSE Monoclonal Antibody, Unconjugated (orb1816767) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with NSE Monoclonal Antibody, Unconjugated (orb1816767) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with NSE Monoclonal Antibody, Unconjugated (orb1816767) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with NSE Monoclonal Antibody, Unconjugated (orb1816767) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with NSE Monoclonal Antibody, Unconjugated (orb1816767) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Quick Database Links

Gene Symbol

ENO2

UniProt

P09104