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NRXN1 Antibody

Catalog Number: orb1246691

DispatchUsually dispatched within 5-10 working days
$ 880.00
Catalog Numberorb1246691
CategoryAntibodies
DescriptionNRXN1 Antibody
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, FC, WB
Predicted ReactivityCanine, Mouse, Rat
ReactivityHuman
ImmunogenThe immunogen for this antibody is: C-KEKQPSSAKSSNKN
Concentration500 ug/mL
Dilution rangePeptide ELISA: antibody detection limit dilution 1:128000.Western Blot:In transfected HEK293 transiently expressing full-length Human NRXN1 (myc and DYKDDDDK tagged), bands of approx. 75 and 60kDa were observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration: 0.5-1ug/ml. Primary incubations were for 1 hour.Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.
Form/AppearanceLiquid
ConjugationUnconjugated
TargetNRXN1
UniProt IDP58400
NCBINP_620072.1, NP_004792.1
StorageAliquot and store at -20˚°C. Minimize freezing and thawing.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesneurexin 1, DKFZp313P2036, NRXN1, FLJ35941, Hs.229
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NoteFor research use only
Application notesPeptide ELISA: antibody detection limit dilution 1:128000.Western Blot:In transfected HEK293 transiently expressing full-length Human NRXN1 (myc and DYKDDDDK tagged), bands of approx. 75 and 60kDa were observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration: 0.5-1ug/ml. Primary incubations were for 1 hour.Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.
Expiration Date12 months from date of receipt.
NRXN1 Antibody

HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human NRXN1 with DYKDDDDK tag probed with orb1246691 (0.5 ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with orb1246691 (1 mg/ml) in Lane B.

NRXN1 Antibody

orb1246691 Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).

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