You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1246691 |
---|---|
Category | Antibodies |
Description | NRXN1 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, FC, WB |
Predicted Reactivity | Canine, Mouse, Rat |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-KEKQPSSAKSSNKN |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:In transfected HEK293 transiently expressing full-length Human NRXN1 (myc and DYKDDDDK tagged), bands of approx. 75 and 60kDa were observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration: 0.5-1ug/ml. Primary incubations were for 1 hour.Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | NRXN1 |
UniProt ID | P58400 |
NCBI | NP_620072.1, NP_004792.1 |
Storage | Aliquot and store at -20˚°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | neurexin 1, DKFZp313P2036, NRXN1, FLJ35941, Hs.229 Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:In transfected HEK293 transiently expressing full-length Human NRXN1 (myc and DYKDDDDK tagged), bands of approx. 75 and 60kDa were observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration: 0.5-1ug/ml. Primary incubations were for 1 hour.Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml. |
Expiration Date | 12 months from date of receipt. |
HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human NRXN1 with DYKDDDDK tag probed with orb1246691 (0.5 ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with orb1246691 (1 mg/ml) in Lane B.
orb1246691 Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).