NRG1 Antibody

• Catalog Number: orb1272653
• Category: Antibodies
• Description: NRG1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, NeA, WB
• Reactivity: Human
• Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Human Heregulin-B1. Human Heregulin-B1 specific antibody was purified by affinity chromatography employing an immobilized Human Heregulin-B1 matrix.
• Concentration: batch dependent
• Dilution range: Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hHeregulin-B1 (1.0 ng/mL), a concentration of 0.1-1.1 μg/mL of this antibody is required.ELISA:In a sandwich ELISA To detect hHeregulin-B1 (using 100 uL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction withour biotinylated Anti-Human hHeregulin-B1as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hHeregulin-B1. Western Blot:To detect hHeregulin-B1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hHeregulin-B1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: NRG1
• UniProt ID: Q02297
• NCBI: Q02297
• Storage: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: GGF, HGL, HRG, NDF, ARIA, GGF2, HRG1, HRGA, SMDF,
• Note: For research use only
• Application notes: Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hHeregulin-B1 (1.0 ng/mL), a concentration of 0.1-1.1 μg/mL of this antibody is required.ELISA:In a sandwich ELISA To detect hHeregulin-B1 (using 100 uL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction withour biotinylated Anti-Human hHeregulin-B1as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hHeregulin-B1. Western Blot:To detect hHeregulin-B1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hHeregulin-B1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To detect Human Heregulin-beta1 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Heregulin-beta1 (orb1272654) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Heregulin-beta1.

To detect Human Heregulin-beta1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human Heregulin-beta1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Human Heregulin-beta1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human Heregulin-beta1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained formalin-fixed, paraffin-embedded sections of human normal breast. The recommended concentration is 0.125 mg/ml-0.0625 mg/ml with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.