Nrf2 (phospho-Ser40) antibody

• Catalog Number: orb6544
• Category: Antibodies
• Description: Nrf2 (phospho-Ser40) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Gallus
• Reactivity: Human, Mouse, Porcine, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Nrf2 around the phosphorylation site of Ser40 (DF(p-S)QR)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, ICC=1:100, Flow-Cyt=1μg /test, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 68 kDa
• Target: phospho-Nrf2 (Ser40)
• UniProt ID: Q16236
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: Nrf2 (phospho S40); p-Nrf2 (phospho S40); Nuclear
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Gupta, Mahesh et al. Dwindling of cardio damaging effect of isoproterenol by Punica granatum L. peel extract involve activation of nitric oxide-mediated Nrf2/ARE signaling pathway and apoptosis inhibition Nitric Oxide, 50, 105-113 (2015)

PubMed: 26363155

Applications

IHC

Reactivity

Rat

Flow cytometric analysis of Jurkat cell lysate using Nrf2 (phospho-Ser40) antibody

IF analysis of HepG2 cell lysate using Nrf2 (phospho-Ser40) antibody

IHC-P image of Mouse brain tissue using Nrf2 (phospho-Ser40) antibody

IHC-P image of Rat brain tissue using Nrf2 (phospho-Ser40) antibody

WB analysis of Lane 1: Hela (Human) Cell Lysate at 30 ug, Lane 2: HepG2 (Human) Cell Lysate at 30 ug, Lane 3: MCF-7 (Human) Cell Lysate at 30 ug, Lane 4: Jurkat (Human) Cell Lysate at 30 ug, Lane 5: B16 (Human) Cell Lysate at 30 ug, Lane 6: A549 (Human) C