NRAMP1/SLC11A1 Antibody

• Catalog Number: orb546332
• Category: Antibodies
• Description: NRAMP1/SLC11A1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human NRAMP1/SLC11A1 recombinant protein (Position: F183-S549).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 90-120 kDa
• UniProt ID: P49279
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Natural resistance-associated macrophage protein 1
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of SLC11A1 using anti-SLC11A1 antibody.Lane 1:human K562 cell;2:human THP-1 cell;3:human placenta tissue;4:human A549 cell;5:human Caco-2 cell;6:human HepG2 cell;7;human U2OS cell;8;human HeLa cell.

IF analysis of SLC11A1 using anti-SLC11A1 antibody. SLC11A1 was detected in immunocytochemical section of MCF-7 cell.

IHC analysis of SLC11A1 using anti-SLC11A1 antibody.SLC11A1 was detected in paraffin-embedded section of human intestinal cancer tissues.

IHC analysis of SLC11A1 using anti-SLC11A1 antibody.SLC11A1 was detected in paraffin-embedded section of mouse spleen tissues.

IHC analysis of SLC11A1 using anti-SLC11A1 antibody.SLC11A1 was detected in paraffin-embedded section of rat spleen tissues.