NR1I3/CAR Antibody

• Catalog Number: orb1538085
• Category: Antibodies
• Description: NR1I3/CAR Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Synthetic peptide located between aa180-229 of human NR1I3 (Q6GZ85, NP_001070939). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Medaka (100%); Zebrafish (85%); Hamster, Turkey, Zebra finch, Chicken, Platypus, Lizard, Stickleback (84%).
• Concentration: 0.5 mg/ml
• Dilution range: IHC, IHC-P (5 µg/ml), WB (0.2 - 1 µg/ml)
• Conjugation: Unconjugated
• Target: NR1I3 / CAR
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 0.09% sodium azide, 2% sucrose. PBS, 0.09% sodium azide, 2% sucrose
• Alternative names: NR1I3, Car-beta, CAR1, Constitutive active recepto
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: LS-B3559 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3559 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.
• Expiration Date: 12 months from date of receipt.