NR1D1 monoclonal antibody (M02), clone 4F6

• Catalog Number: orb2291698
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant NR1D1.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1 Kappa
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Immunogen: NR1D1 (AAH56148, 1 a.a. ~ 614 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: MTTLDSNNNTGGVITYIGSSGSSPSRTSPESLYSDNSNGSFQSLTQGCPTYFPPSPTGSLTQDPARSFGSIPPSLSDDGSPSSSSSSSSSSSSFYNGSPPGSLQVAMEDSSRVSPSKSTSNITKLNGMVLLCKVCGDVASGFHYGVHACEGCKGFFRRSIQQNIQYKRCLKNENCSIVRINRNRCQQCRFKKCLSVGMSRDAVRFGRIPKREKQRMLAEMQSAMNLANNQLSSQCPLETSPTQHPTPGPMGPSPPPAPVPSPLVGFSQFPQQLTPPRSPSPEPTVEDVISQVARAHREIFTYAHDKLGSSPGNFNANHASGSPPATTPHRWENQGCPPAPNDNNTLAAQRHNEALNGLRQAPSSYPPTWPPGPAHHSCHQSNSNGHRLCPTHVYAAPEGKAPANSPRQGNSKNVLLACPMNMYPHGRSGRTVQEIWEDFSMSFTPAVREVVEFAKHIPGFRDLSQHDQVTLLKAGTFEVLMVRFASLFNVKDQTVMFLSRTTYSLQELGAMGMGDLLSAMFDFSEKLNSLALTEEELGLFTAVVLVSADRSGMENSASVEQLQETLLRALRALVLKNRPLETSRFTKLLLKLPDLRTLNNMHSEKLLSFRVDAQ
• Tested applications: ELISA, IF, IHC-P, WB
• Clone Number: 4F6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• NCBI: AAH56148

Detection limit for recombinant GST tagged NR1D1 is approximately 1 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to NR1D1 on HeLa cell. [antibody concentration 10 ug/ml]

Immunoperoxidase of monoclonal antibody to NR1D1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]

NR1D1 monoclonal antibody (M02), clone 4F6 Western Blot analysis of NR1D1 expression in Hela S3 NE.

NR1D1 monoclonal antibody (M02), clone 4F6. Western Blot analysis of NR1D1 expression in NIH/3T3.

Western blot analysis of NR1D1 over-expressed 293 cell line, cotransfected with NR1D1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NR1D1 monoclonal antibody (M02), clone 4F6 (Cat # orb2291698). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (93.28 KDa).