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Catalog Number | orb1526022 |
---|---|
Category | Antibodies |
Description | NPAT Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP |
Reactivity | Human |
Immunogen | between 700 and 750 |
Concentration | 1000 µg/ml |
Dilution range | WB - Not recommended. Use rabbit anti-NPAT antibody A302-772A.; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | NPAT |
UniProt ID | Q14207 |
NCBI | NP_002510.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | nuclear protein of the ataxia telangiectasia mutat Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human NPAT by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-NPAT antibody orb1526022 used for IP at 3 µg/mg lysate.
Detection of human NPAT by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-NPAT (Cat. No. orb1526022) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
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