NOX5 antibody

• Catalog Number: orb125115
• Category: Antibodies
• Description: Goat polyclonal antibody to NOX5
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Bovine, Canine, Human
• Dilution range: ELISA: 1:8000, WB: 0.5-2 μg/ml, IHC-P: 10ug/ml
• Conjugation: Unconjugated
• MW: 86.4; 83.8; 83.1
• Target: NOX5
• Entrez: 79400
• Protein Sequence: EWHPFTISSAPEQKD
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti NOX5 antibody, anti NADPH oxidase, EF-hand ca
• Note: For research use only
• Application notes: WB: Approx 90kDa band observed in Human Testis lysates (calculated MW of 86.4kDa according to NP_078781.3). Recommended concentration: 0.5-2µg/ml. Primary incubation 1 hour at room temperature.. Notes: Immunofluorescence: Strong expression of the protein seen in the plasma membrane and cytoplasm of HeLa and MCF7 cells. Recommended concentration:10ug/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Human Testis lysate using NOX5 antibody

Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells using NOX5 antibody

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using NOX5 antibody