NOX4 Antibody

• Catalog Number: orb1239341
• Category: Antibodies
• Description: NOX4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: NOX4 antibody was raised against a 14 amino acid peptide near the amino terminus of human NOX4.The immunogen is located within amino acids 70 - 120 of NOX4.
• Concentration: 1 mg/mL
• Dilution range: NOX4 antibody can be used for detection of NOX4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 64 kDa Observed: 68 kDa
• Target: NOX4
• UniProt ID: Q9NPH5
• NCBI: NP_058627
• Storage: NOX4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
• Buffer/Preservatives: NOX4 antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: NAPDH oxidase 4, NOX-4, RENOX, KOX, KOX-1
• Note: For research use only
• Application notes: NOX4 antibody can be used for detection of NOX4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NOX4 in Jurkat cell lysate with NOX4 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry of NOX4 in human spleen tissue with NOX4 antibody at 5 µg/ml.

Immunofluorescence of NOX4 in human spleen tissue with NOX4 antibody at 20 µg/ml.