The Novel Green Plus provides an easy 2-step method to stain the DNA band from DNA electrophresis. This unique reagent ensures the DNA to be stained with a high sensitivity and good quality on the gel. Novel Green Plus is a next-generation DNA-binding dye with features ideal for use in quantitative real-time PCR (qPCR) and many other applications. We designed the dye by taking into consideration several essential dye properties relevant to PCR, including PCR inhibition, safety, and stability and fluorescence spectra of the dye. Ethidium bromide (EtBr), which presents sensitivity for detecting 1-5 ng double-stranded DNA (dsDNA) in the agarose gel analysis, has been the most common dye used for nucleic acid gel staining.