NOV/CCN3 Antibody

• Catalog Number: orb402566
• Category: Antibodies
• Description: NOV/CCN3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human NOV/CCN3 (334-357aa HTNCPKNNEAFLQELELKTTRGKM), different from the related mouse sequence by seven amino acids, and from the related rat sequence by four amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 39162 MW
• UniProt ID: P48745
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Protein NOV homolog;NovH;CCN family member 3;Insul
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U87 cells using anti-NOV/CCN3 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of NOV/CCN3 using anti-NOV/CCN3 antibody.Lane 1:human HeLa Cell.

IF analysis of NOV/CCN3 using anti-NOV/CCN3 antibody. NOV/CCN3 was detected in immunocytochemical section of A431 cells.

IHC analysis of NOV/CCN3 using anti-NOV/CCN3 antibody.NOV/CCN3 was detected in paraffin-embedded section of rat kidney tissue.

IHC analysis of NOV/CCN3 using anti-NOV/CCN3 antibody.NOV/CCN3 was detected in paraffin-embedded section of human mammary cancer tissue.

IHC analysis of NOV/CCN3 using anti-NOV/CCN3 antibody.NOV/CCN3 was detected in paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of NOV/CCN3 using anti-NOV/CCN3 antibody.NOV/CCN3 was detected in paraffin-embedded section of human lung cancer tissue.