NOTCH2 antibody

• Catalog Number: orb750483
• Category: Antibodies
• Description: NOTCH2 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: Antiserum
• Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acid residues 2390-2415 of human Notch 2 (the total protein is 2471 aa). A residue of cysteine was added to the amino terminal end to facilitate coupling.
• Concentration: 75 mg/mL
• Dilution range: ELISA: 1:30,000 - 1:90,000, IHC: 1:200-1:800, WB: 1:400 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This antiserum is directed against human NOTCH 2. The peptide sequence shows 100% alignment with human, dog and chimpanzee sequence. Only one (1) amino acid difference is found in mouse and this change is non-conservative. Based on the sequence we expect this antibody to react as well with rat and mouse NOTCH 2. No specific information is available for other reactivities.
• Conjugation: Unconjugated
• UniProt ID: Q04721
• NCBI: 24041035
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Alternative names: rabbit anti-Notch 2 antibody, AGS 2 antibody, AGS2
• Note: For research use only
• Application notes: This antibody has been tested for use in ELISA and western blot. For western blot experiments, one can expect a band of ≈110 kDa in size corresponding to active Notch 2 in the appropriate cell lysate. A 1:500 dilution was effective for staining FFPE human kidney tissue by Immunohistochemistry (IHC). Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.