NOS3 (phospho-S1179) antibody

• Catalog Number: orb515508
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NOS3 (phospho-S1179)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S1179 of human eNOS protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: NOS3
• Entrez: 24600, 4846, 18127
• UniProt ID: P70313, Q62600, P29474
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Nitric oxide synthase endothelial; Constitutive NO
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of eNOS (Phospho-S1179) expression in mouse spleen (A), mouse liver (B), rat spleen (C) whole cell lysates. (Predicted band size: 133; 69 kD; Observed band size: 140 kD)

Immunohistochemical analysis of eNOS (Phospho-S1179) staining in human tonsil cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.