NOS1 (phospho-S1417) antibody

• Catalog Number: orb515268
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NOS1 (phospho-S1417)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S1417 of human nNOS protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: NOS1
• Entrez: 18125, 24598, 4842
• UniProt ID: P29476, Q9Z0J4, P29475
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Nitric oxide synthase brain; Constitutive NOS; NC-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of nNOS (Phospho-S1417) expression in A549 (A), HEK293T (B) whole cell lysates. (Predicted band size: 160 kD; Observed band size: 160 kD)

Immunohistochemical analysis of nNOS (Phospho-S1417) staining in human stomach cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.