NOPP140 Antibody

• Catalog Number: orb1527064
• Category: Antibodies
• Description: NOPP140 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human
• Immunogen: between 1 and 50
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended. Use rabbit anti-NOPP140 antibody A302-185A.; IP - 5 - 15 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: NOPP140
• UniProt ID: Q14978
• NCBI: NP_004732.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: nucleolar 130 kDa protein; P130; nucleolar protein
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human NOPP140 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from K-562 cells. Antibodies: Affinity purified rabbit anti-NOPP140 antibody orb1527064 used for IP at 3 µg/mg lysate.

Detection of human NOPP140 by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-NOPP140 (Cat. No. orb1527064) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB