NOPP140 Antibody

• Catalog Number: orb1527061
• Category: Antibodies
• Description: NOPP140 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: between 580 and 630
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 5 - 15 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: NOPP140
• UniProt ID: Q14978
• NCBI: NP_004732.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: nucleolar 130 kDa protein; P130; nucleolar protein
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human NOPP140 by western blot and immunoprecipitation. Samples: Whole cell lysate from K-562 (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HeLa (H; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NOPP140 antibody orb1527061 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. NOPP140 was also immunoprecipitated by rabbit anti-NOPP140 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).