NONO Antibody

• Catalog Number: orb1529950
• Category: Antibodies
• Description: NONO Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: between 350 and 400
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: NONO
• UniProt ID: Q15233
• NCBI: NP_031389.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: PPP1R114; protein phosphatase 1, regulatory subuni
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human NONO by western blot.Samples: Whole cell lysate from HEK293T, HeLa, Jurkat, MCF-7, and Hep-G2 cells. Antibody: Affinity purified rabbit anti-NONO antibody at 0.04 µg/ml.

Detection of mouse and rat NONO by western blot.Samples: Whole cell lysate from NIH 3T3, TCMK-1, 4T1, CT26, and C6 cells.Antibody: Affinity purified rabbit anti-NONO antibody at 0.04 µg/ml.

Detection of human NONO by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-NONO used at a dilution of 1:1, 000 (0.2 µg/ml).

Detection of mouse NONO by immunohistochemistry. Sample: FFPE section of mouse intestine. Antibody: Affinity purified rabbit anti-NONO used at a dilution of 1:200 (1 µg/ml).

Detection of human NONO by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-NONO antibody.