Non-structural protein 10 Antibody

• Catalog Number: orb1239080
• Category: Antibodies
• Description: Non-structural protein 10 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Virus
• Isotype: IgG
• Immunogen: Anti-SARS-CoV-2 (COVID-19) NSP10 antibody (orb1239080) was raised against a peptide corresponding to 10 amino acids near the carboxyl terminus of SARS-CoV-2 (COVID-19) NSP10 protein. The immunogen is located in the last 50 amino acids of SARS-CoV-2 (COVID-19) NSP10.
• Concentration: 1 mg/mL
• Dilution range: WB 1-4 μg/mL; IHC: 0.5 μg/mL. Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) NSP10 antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. It can detect SARS-CoV-2 NSP10 recombinant protein by ELISA and WB. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: Non-structural protein 10
• UniProt ID: P0DTC1
• NCBI: YP_009742617.1
• Storage: SARS-CoV-2°COVID-19) NSP10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: SARS-CoV-2 (COVID-19) NSP10 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: NSP10, Growth factor-like peptide, GFL
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) NSP10 in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) NSP10 antibody (orb1239080, 0.5 µg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP10 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.

ELISA Validation with SARS-CoV-2 (COVID-19) NSP10 Protein. Antibodies: SARS-CoV-2 (COVID-19) NSP10 Antibody, orb1239080 A direct ELISA was performed using SARS-CoV-2 NSP10 recombinant protein (orb1231581) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP10 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL.

Western Blot Validation with SARS-CoV-2 (COVID-19) NSP10 Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) NSP10 recombinant protein (orb1231581). Antibodies: SARS-CoV-2 (COVID-19) NSP10, orb1239080, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 µg/mL. Lane 2: 2 µg/mL. Lane 3: 4 µg/mL.