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Noggin Rabbit Polyclonal Antibody

Catalog Number: orb100968

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb100968
CategoryAntibodies
DescriptionNoggin Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC-Fr, IHC-P
Predicted ReactivityBovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human Noggin (28-130/232aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW23 kDa
TargetNOG
UniProt IDQ13253
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesNOG; SYM 1; SYM1; Symphalangism 1(proximal); Synos
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NoteFor research use only
Expiration Date12 months from date of receipt.
Noggin Rabbit Polyclonal Antibody

Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-Noggin antibody (orb100968), Dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Negative control (white blue line): PBS, Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Noggin Rabbit Polyclonal Antibody

Tissue/cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Noggin Polyclonal Antibody, Unconjugated (orb100968) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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