NOC4L Antibody

• Catalog Number: orb2636889
• Category: Antibodies
• Description: Treg activation is critical for maintaining self-tolerance, but the translational control of this process is still poorly understood. Zhu et al. report a conserved ribosome biogenesis factor, Noc4L, that regulates mRNAs related to Treg and Tconv activation but does not affect global protein translation. Noc4L regulates translation of mRNAs related to Treg activation. Noc4L plays critical roles in activation of Tregs and Tconvs. Noc4L-mediated ribosome biogenesis is critical in controlling the activation of Tregs and maintaining immune tolerance. [Zhu et al., 2019, Cell Reports 27, 1205 1220.]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-NOC4L-1E3
• Tested applications: FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Recombinant full-length human NOC4L protein was used as the immunogen for the NOC4L antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This NOC4L antibody is available for research use only.
• UniProt ID: Q9BVI4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the NOC4L antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human HeLa cells using NOC4L antibody (green, clone PCRP-NOC4L-1E3) and phalloidin (red).

Immunofluorescent staining of PFA-fixed human HeLa cells NOC4L antibody (green, clone PCRP-NOC4L-1E3) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with NOC4L antibody (blue, clone PCRP-NOC4L-1E3), and unstained cells (red).

SDS-PAGE analysis of purified, BSA-free NOC4L antibody (clone PCRP-NOC4L-1E3) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using NOC4L antibody (clone PCRP-NOC4L-1E3). These results demonstrate the foremost specificity of the PCRP-NOC4L-1E3 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.