NME1 Antibody / NM23

• Catalog Number: orb2643605
• Category: Antibodies
• Description: The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP-K). In humans, NDP kinase A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease hypersensitive elements at positions 142 to 115 of the human C-Myc promotor.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CPTC-NME1-2
• Tested applications: WB
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: A recombinant full-length human NME1 protein was used as the immunogen for this NME1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This NME1 antibody is available for research use only.
• UniProt ID: P15531
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human HeLa cell lysate with NME1 antibody. Predicted molecular weight ~17 kDa.

SDS-PAGE analysis of purified, BSA-free NME1 antibody as confirmation of integrity and purity.