| Catalog Number | orb157946 |
|---|---|
| Tested applications | ELISA, ICC, IF, WB, IHC |
| Reactivity | Bovine, Gallus, Human, Mouse, Porcine, Rabbit, Sheep |
| Storage | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| Description | Rabbit polyclonal antibody to NLK |
| Alternative names | Nemo-like kinase (Phospho-Thr298), Nemo-like kinase (Phospho-T298), p-Nemo-like kinase (Thr298), p-Nemo-like kinase (T298), DKFZp761G1211, FLJ21033, LAK1, Nemo like kinase, Nemo-like kinase, Nlk, NLK_HUMAN, Protein LAK1, Serine/threonine protein kinase NLK, Serine/threonine-protein kinase NLK.(click to expand) |
| Expiration Date | 12 months from date of receipt. |
| Note | For research use only. |
- Wilkes, M. C. et al. Diamond Blackfan anemia is mediated by hyperactive Nemo-like kinase Nat Commun, 11, 3344 (2020)
Sample:
Cerebral cortex (Mouse) Lysate at 40 ug
Primary: Anti- Phospho-Nemo-like kinase (Thr298)at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for
- NLK antibody (Biotin) [orb502571]
ELISA, ICC, IF, IHC-P, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Biotin
100 μl
