NLK (Phospho-Thr298) antibody

• Catalog Number: orb157946
• Category: Antibodies
• Description: NLK (Phospho-Thr298) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Bovine, Gallus, Porcine, Rabbit, Rat, Sheep
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human Nemo-like kinase around the phosphorylation site of Thr298 (HM(p-T)QE)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 58 kDa
• Target: Phospho-NLK (Thr298)
• UniProt ID: Q9UBE8
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: Nemo-like kinase (Phospho-Thr298); Nemo-like kinas
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Wilkes, M. C. et al. Diamond Blackfan anemia is mediated by hyperactive Nemo-like kinase Nat Commun, 11, 3344 (2020)

PubMed: 32620751

Sample: Cerebral cortex (Mouse) Lysate at 40 ug Primary: Anti- Phospho-Nemo-like kinase (Thr298)at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for