NKX2-5 Antibody

• Catalog Number: orb1249612
• Category: Antibodies
• Description: NKX2-5 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Predicted Reactivity: Bovine, Canine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-PRAYSDPDPAKDPR
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40-45kDa band observed in Mouse Heart lysates (calculated MW of 34.2kDa according to NP_032726.1). Preliminary experimants also showed an apprix 45kDa band in Human and Rat Heart kysates. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Shiojima et al, Circ Res. 1996 Nov;79(5):920-9.; PMID: 8888684). Recommended concentration: 0.1-0.5ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of U2OS cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NKX2-5
• UniProt ID: P52952
• NCBI: NP_001159647.1, NP_001159648.1, NP_004378.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: NKX2-5, NK2 transcription factor related, locus 5
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40-45kDa band observed in Mouse Heart lysates (calculated MW of 34.2kDa according to NP_032726.1). Preliminary experimants also showed an apprix 45kDa band in Human and Rat Heart kysates. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Shiojima et al, Circ Res. 1996 Nov;79(5):920-9.; PMID: 8888684). Recommended concentration: 0.1-0.5ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of U2OS cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249612 (0.3 ug/ml) staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249612 (3.75 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1249612 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining.