Nitrotyrosine antibody

• Catalog Number: orb147403
• Category: Antibodies
• Description: Mouse monoclonal to Nitrotyrosine (RPE). Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosino-philperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury. The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimers disease, Parkinsons disease and amyotrophic lateral sclerosis..
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 39B6
• Tested applications: AM, ELISA, FC, ICC, IHC, IP, WB
• Reactivity: All
• Isotype: IgG2a
• Immunogen: 3-(4-hydroxy-3-nitrophenylacetamido) propionic acid-bovine serum albumin
• Concentration: 1 mg/ml
• Dilution range: WB (1:1400), IHC (1:100)
• Conjugation: RPE
• Target: Nitrotyrosine
• Storage: Conjugated antibodies should be stored at 4°C
• Buffer/Preservatives: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
• Alternative names: Nitro tyrosine antibody, 3-Nitrotyrosine antibody
• Note: For research use only
• Application notes: 0.7 µg/ml of SMC-154 was sufficient for detection of 5 µg SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.