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Nitric Oxide Colorimetric Detection Kit

Nitric Oxide Colorimetric Detection Kit

Catalog Number: orb511103

DispatchUsually dispatched within 5-10 working days
$ 660.00
Catalog Numberorb511103
CategoryTools
DescriptionThis kit offers two assays. The first quantitatively measures endogenous Nitrite. In the second, Nitrate is converted to Nitrite using Nitrate Reductase and Total Nitric Oxide is measured. Nitrate levels can be calculated by performing both assays.
TargetNitric Oxide
Storage2-8°C
NoteFor research use only
Application notes1. Prepare samples and controls., 2. Reconstitute ThioBright Green with 500 µL DMSO to produce a 200X stock concentrate., 3. Add ThioBright Green to each sample at 1:200. For example, add 5 µL to 995 µL cultured cells., 4. Incubate 15-30 minutes., 5. Optional: Spin and pellet cells, remove supernatants, and gently resuspend in Assay Buffer or other isotonic buffer., 6. If desired, label with additional stains, such as Hoechst, Propidium Iodide, 7-AAD, or an antibody., 7. Analyze with a flow cytometer. ThioBright Green excites at 490 nm and emits at 525 nm.
Sample TypesWater, buffers, serum, plasma, urine, saliva, tissue
Expiration Date12 months from date of receipt.