NFYB Antibody

• Catalog Number: orb1268385
• Category: Antibodies
• Description: NFYB Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Predicted Reactivity: Bovine, Gallus, Mouse, Rat
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This NFYB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human NFYB.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 23 kDa
• Target: NFYB
• UniProt ID: P25208
• NCBI: P25208
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Nuclear transcription factor Y subunit beta, CAAT
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis in A549 cell line lysates (35 ug/lane).

Fluorescent confocal image of Hela cell stained with NFYB Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFYB primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NFYB immunoreactivity is localized to nucleus significantly.