NFkB (phospho-50) ELISA Kit

• Catalog Number: orb343759
• Category: Assays and Kits
• Description: NFkB (phospho-50) ELISA Kit
• Tested applications: ELISA
• Reactivity: Human
• Concentration: 1 Kit
• Dilution range: ELISA: 1:100
• Conjugation: Unconjugated
• Storage: See kit insert for complete instructions.
• Alternative names: NF-kB Transcription Factor Kit, p50 kit, EIA kit,
• Note: For research use only
• Application notes: Rockland's NF-kB (p50) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-kB response element is immobilized onto the bottom of wells of a 96 well plate (see Figure 1 on page 4). NF-kB contained in a nuclear extract specifically binds to the NF-kB response element. NF-kB (p50) is detected by addition of a specific primary antibody directed against NF-kB (p50). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. Rockland's NF-kB (p50) Transcription Factor Assay detects human NF-kB (p50). It will not cross-react with NF-kB (p65).
• Expiration Date: Please enquire.

ELISA of Transcription Factor-Transcription factor assay absorbance of cell lysates isolated from stimulated (20 ng/mL TNFa for 30 min.) and non-stimulated HeLa cells demonstrating NF-kB (p50) activity.

Sample Plate Format