NFKB p65 (phospho-Thr435) antibody

• Catalog Number: orb6506
• Category: Antibodies
• Description: NFKB p65 (phospho-Thr435) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human NFKBp65 around the phosphorylation site of Thr435 (EG(p-T)LS)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, ICC=1:100, Flow-Cyt=2ug/Test, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 61 kDa
• Target: phospho-NFKB p65 (Thr435)
• UniProt ID: Q04206
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: NF-kB p65 (phospho T435); p-NF-kB p65 (phospho T43
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry of Mouse spleen.Primary Antibody (green line): Rabbit Anti-phospho-NFKB p65 (Thr435) antibody; Dilution:2μg /10^6 cells.

Flow cytometry of MCF7.Primary Antibody (green line): Rabbit Anti-phospho-NFKB p65 (Thr435) antibody; Dilution:2μg /10^6 cells.

ICC analysis of Hela cell using phospho-NFKB p65 (Thr435) polyclonal Antibody at 1:100 dilution;DAPI (blue) was used to stain the cell nuclei.